10×Transcription Buffer |
100 μl |
T7 RNA Polymerase (50 U/μl) |
100 μl |
RNase Inhibitor (40 U/μl) |
25 μl |
ATP Solution (50 mM) |
100 μl |
GTP Solution (50 mM) |
100 μl |
CTP Solution (50 mM) |
100 μl |
UTP Solution (50 mM) |
100 μl |
RNase free DNase I (5 U/μl) |
200 μl |
Control Template (50 ng/μl) |
20 μl |
RNase T1 (100 U/μl) |
25 μl |
RNase T1 Dilution Buffer |
700 μl |
RNase free dH2O |
1 ml |
10×Annealing Buffer* |
500 μl |
|
* 二条Oligo DNA退火时使用。 |
Buffer组成:100 mM Tris-HCl (pH8.0),500 mM CH3COOK,10 mM EDTA。 |